基因Ⅰ型猪乙型脑炎灭活疫苗免疫原性评价

doi:10.11843/j.issn.0366-6964.2017.05.013

Abstract

Abstract:

The objective of this study was to evaluate the immunogenicity of inactivated vaccines of swine Japanese encephalitis virus genetype Ⅰ of YA1 and CZ strains. High titer viruses were obtained by serial passages on suckling mouse brains. Then inactivated vaccines of YA1 and CZ strains were prepared by mixing these inactivated viruses with adjuvant ISA206, respectively. Furthermore, the immunogenicity of inactivated vaccines were evaluated in mice, guinea pigs and piglets. IgG antibody, hemagglutination inhibition antibody, plaque reduction neutralizing antibody and vaccine protection rate were detected through indirect ELISA test, hemagglutination inhibition test, plaque reduction neutralization test and protection rate test, respectively. These results showed that inactivated vaccines of YA1 and CZ strains produced higher IgG antibody, hemagglutination inhibition antibody, plaque reduction neutralizing antibody, and vaccine protective rate than HW1 strain in these experimental animals. These results indicated that the immunogenicity of inactivated vaccines of YA1, CZ strains was better than inactivated vaccine of HW1 strain. Above all, the immunogenicity of the inactivated vaccine of CZ strain was the best.

CLC Number:

S813.1

FENG Yao, YUE Hui-xi, LIU Hong-ming, CAO San-jie, WU Rui, HUANG Xiao-bo, WEN Yi-ping, ZHAO Qin, WEN Xin-tian. The Immunogenicity Evaluation of Inactivated Vaccines of Swine Japanese Encephalitis Virus Genetype Ⅰ[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 889-895.

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The Immunogenicity Evaluation of Inactivated Vaccines of Swine Japanese Encephalitis Virus Genetype Ⅰ

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